Journal: BMC Oral Health
Article Title: Dose-dependent effects of Metformin on proliferation and odontogenic differentiation of dental pulp stem cells
doi: 10.1186/s12903-026-08221-w
Figure Lengend Snippet: Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model TCM400 and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain
Article Snippet: Images were captured using the LABOMED Trinocular inverted phase contrast microscope model TCM400 and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14.
Techniques: Staining, Cell Culture, Control, Microscopy, Software, Derivative Assay